4H5P

Crystal Structure of Rift Valley Fever Virus Nucleocapsid Protein Tetramer Bound to Single-stranded RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	77.640, 193.180, 77.420
Unit cell angles	90.00, 108.89, 90.00

Refinement procedure

Resolution	31.480 - 2.150
R-factor	0.1822
Rwork	0.180
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lyf
RMSD bond length	0.009
RMSD bond angle	0.930
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	96.590	48.423	2.270
High resolution limit [Å]	2.150	6.800	2.150
Rmerge		0.045	0.584
Total number of observations		7092	21896
Number of reflections	55733
<I/σ(I)>	9.3	6.6	1.3
Completeness [%]	95.5	99.1	77.9
Redundancy	3.7	3.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	18% PEG3350, 350 mM sodium chloride, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K