4H5J

Crystal Structure of the Guanine Nucleotide Exchange Factor Sec12 (P64 form)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792, 0.9794, 0.9640
Spacegroup name	P 64
Unit cell lengths	189.473, 189.473, 53.424
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.368 - 2.601
R-factor	0.1807
Rwork	0.179
R-free	0.21640
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.189
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX (dev_1144)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.120	0.072	0.446
Number of reflections	34159
<I/σ(I)>	7.6
Completeness [%]	99.9	99.7	99.8
Redundancy	8.3	8.9	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.3	298	91 mM Tris, pH 8.0, 181 mM potassium citrate, pH 8.3, 22% w/v PEG3350, VAPOR DIFFUSION, temperature 298K