4H5I

Crystal Structure of the Guanine Nucleotide Exchange Factor Sec12 (P1 form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 1
Unit cell lengths	53.400, 53.474, 61.976
Unit cell angles	81.68, 73.37, 87.58

Refinement procedure

Resolution	42.359 - 1.360
R-factor	0.1795
Rwork	0.179
R-free	0.19710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.248
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_1144)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.370
High resolution limit [Å]	1.350	3.660	1.350
Rmerge	0.063	0.036	0.610
Number of reflections	132685
<I/σ(I)>	11.2
Completeness [%]	93.6	88.1	79.8
Redundancy	3.8	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	100 mM Tris, pH 8.0, 400 mM potassium chloride, 22% w/v PEG3350, VAPOR DIFFUSION, temperature 298K