4H5A
DNA octamer D (GTseGTACAC) partially crosslinked with platinum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2012-07-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.310, 42.310, 24.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.080 - 1.420 |
| R-factor | 0.21317 |
| Rwork | 0.212 |
| R-free | 0.23708 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 3.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.080 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.091 | 0.584 |
| Number of reflections | 4265 | |
| <I/σ(I)> | 17.93 | 3.73 |
| Completeness [%] | 99.5 | |
| Redundancy | 12.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | the platinum was added to the crystallization buffer , pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
