4H56
Crystal structure of the Clostridium perfringens NetB toxin in the membrane inserted form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-12 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 313.230, 168.040, 160.460 |
| Unit cell angles | 90.00, 109.42, 90.00 |
Refinement procedure
| Resolution | 27.700 - 3.900 |
| R-factor | 0.2851 |
| Rwork | 0.284 |
| R-free | 0.30890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ahl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.832 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (MR) |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 4.110 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.344 | 0.685 |
| Number of reflections | 69515 | |
| <I/σ(I)> | 3.7 | 2 |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289.15 | 12% PEG 4000, 100 mM Sodium cacodylate, 0.1 mM Polyethylene glycol dodecyl ether , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289.15K |
