4H55
Crystal structure of Canavalia brasiliensis seed lectin (ConBr) in complex with beta-d-ribofuranose
Replaces: 4DPSExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Detector technology | CCD |
| Collection date | 2006-09-25 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.428 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.240, 70.430, 97.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.390 - 2.150 |
| R-factor | 0.18528 |
| Rwork | 0.183 |
| R-free | 0.22657 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.142 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.680 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 12586 |
| Completeness [%] | 90.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.8 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M HEPES, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
