4H4R
Crystal Structure of Ferredoxin reductase, BphA4 E175C/Q177G mutant (oxidized form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-11-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98008 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 98.456, 98.456, 170.721 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.325 - 1.400 |
R-factor | 0.1985 |
Rwork | 0.198 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gqw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.169 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.330 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 95862 | |
Completeness [%] | 99.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 2.5M Sodium formate, 0.1M acetate buffer, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |