4H4Q

Crystal Structure of Ferredoxin reductase, BphA4 E175Q/Q177K (reduced form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-10-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	97.868, 97.868, 170.568
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.855 - 1.950
R-factor	0.2069
Rwork	0.206
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yvf
RMSD bond length	0.007
RMSD bond angle	1.176
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860	2.060
High resolution limit [Å]	1.950	1.950
Number of reflections	34855
Completeness [%]	97.1	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	293	2.5M Sodium formate, 0.1M acetate buffer, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K