4H4K
Structure of the Cmr2-Cmr3 subcomplex of the Cmr RNA-silencing complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-07-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.04560 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.523, 135.958, 189.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.833 - 2.804 |
| R-factor | 0.2185 |
| Rwork | 0.214 |
| R-free | 0.28380 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 32764 | |
| Completeness [%] | 99.7 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 303.15 | 20% Polyethylene Glycol (PEG1500) , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303.15K |
