4H45
Product Complexes of Porcine Liver Fructose-1,6-bisphosphatase with Mutation E192Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-11 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.996, 83.621, 166.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.420 - 3.100 |
| R-factor | 0.22921 |
| Rwork | 0.227 |
| R-free | 0.28270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cnq |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.855 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK (9.7L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.420 | 32.420 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.450 | 3.000 |
| Rmerge | 0.129 | 0.051 | 0.426 |
| Number of reflections | 7665 | ||
| <I/σ(I)> | 6.9 | 16.4 | 2.3 |
| Completeness [%] | 97.3 | 90.3 | 99.9 |
| Redundancy | 3.49 | 3.54 | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, 1,2-ethanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
