4H44

2.70 A Cytochrome b6f Complex Structure From Nostoc PCC 7120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Collection date	2007-11-25
Detector	ADSC QUANTUM 315
Spacegroup name	P 61 2 2
Unit cell lengths	159.127, 159.127, 364.373
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.547 - 2.700
R-factor	0.2208
Rwork	0.220
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2ZT9
RMSD bond length	0.004
RMSD bond angle	0.997
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	39.550
High resolution limit [Å]	2.700
Number of reflections	74395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K