4H43

Structure of the Salmonella plasmid virulence C protein (SpvC) H106N mutant.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	80
Detector technology	CCD
Collection date	2012-02-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97591
Spacegroup name	P 32
Unit cell lengths	70.989, 70.989, 142.972
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.494 - 2.304
R-factor	0.1767
Rwork	0.175
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b06
RMSD bond length	0.007
RMSD bond angle	0.980
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.076	0.614
Number of reflections	35594
<I/σ(I)>	33.7	4.5
Completeness [%]	99.8	99.5
Redundancy	11.4	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	20% PEG 550 MME, 10% PEG 20k, 0.1 M Amino Acid Mix, 0.1 M Buffer System 1 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K