4H3V
Crystal structure of oxidoreductase domain protein from Kribbella flavida
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97931 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.597, 70.597, 288.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.235 - 1.680 |
R-factor | 0.1443 |
Rwork | 0.144 |
R-free | 0.17740 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.391 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: dev_1096)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.090 | 0.590 |
Number of reflections | 84453 | |
<I/σ(I)> | 21.9 | 2.59 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 297 | 0.1 M Mg formate, 19% PEG3350, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 297K |