4H3T
Crystal structure of CRISPR-associated protein Cse1 from Acidimicrobium ferrooxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 137.017, 137.017, 203.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.720 - 2.030 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.19910 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.070 | 0.730 |
| Number of reflections | 62132 | |
| <I/σ(I)> | 25.3 | 2.4 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 12 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M proline, 0.1 M HEPES/NaOH, 10% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
