4H3L
mPlum-E16P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2012-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.5418, 1.5621, 1.7321 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.229, 76.888, 95.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.870 - 1.650 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qlg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.440 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.730 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.082 | 0.511 |
| Number of reflections | 53773 | |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 97.9 | 97.5 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.3 uL + 0.3 uL drops, precipitant: 200 mM magnesium chloride, 100 mM sodium cacodylate, pH 6.5, 50% (v/v) PEG200, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
