4H33
Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes, tetragonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0330 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 53.370, 53.370, 108.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.400 - 3.100 |
| R-factor | 0.2845 |
| Rwork | 0.281 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eff 2atk 2A79 and 1ORQ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.132 | 0.920 |
| Number of reflections | 2994 | |
| <I/σ(I)> | 18.6 | 2.45 |
| Completeness [%] | 94.4 | 75 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 26-30% (v/v) PEG-MME-550, 0.5 M to 0.6 M ammonium sulfate, and 50 mM ADA, Lipidic Cubic Phase (LCP) , pH 7.0, temperature 293K |
