4H32

The crystal structure of the hemagglutinin H17 derived the bat influenza A virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	74.149, 101.840, 497.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.884 - 2.700
R-factor	0.2191
Rwork	0.217
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.949
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Completeness [%]	92.8	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289	Protein (1ul at 10 mg/ml) in 20 mM Tris (pH 8.0) and 50 mM NaCl was mixed with 1ul reservoir solution (0.1 M sodium citrate tribasic dehydrate (pH 5.0) and 30% v/v Jeffamine, ED-2001 (pH 7.0), VAPOR DIFFUSION, SITTING DROP, temperature 289K