4H2L

Deer mouse hemoglobin in hydrated format

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2012-07-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	76.675, 80.061, 86.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.433 - 1.779
R-factor	0.165
Rwork	0.162
R-free	0.20110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.064
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.433	1.900
High resolution limit [Å]	1.779	1.700
Completeness [%]	98.5	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	299	28% (w/v) PEG 3350 in 50 mM sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 299K