4H0X
Crystal structure of NAD+-Ia(E380A)-actin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.078, 134.654, 152.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.580 - 2.330 |
| R-factor | 0.22575 |
| Rwork | 0.224 |
| R-free | 0.25111 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h03 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.343 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.055 | 0.204 |
| Number of reflections | 47500 | |
| <I/σ(I)> | 24.9 | 9.6 |
| Completeness [%] | 97.3 | 80 |
| Redundancy | 6.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.13 | 18% PEG1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.13K |
