4H0M
X-Ray Crystal Structure of Phycocyanin from Synechococcus elongatus sp. PCC 7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.840, 113.520, 184.440 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.200 |
| R-factor | 0.24464 |
| Rwork | 0.242 |
| R-free | 0.29487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.259 |
| Number of reflections | 195069 | |
| <I/σ(I)> | 7.5 | 3 |
| Completeness [%] | 87.7 | 90.5 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 4000, 50mM tris pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
