4H0H
Crystal structure of mimicry-recognizing 2D10 scFv with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.330, 80.330, 74.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.500 - 2.000 |
| R-factor | 0.19725 |
| Rwork | 0.195 |
| R-free | 0.21525 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h0i |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.865 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.568 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.427 |
| Number of reflections | 19310 | |
| <I/σ(I)> | 24.1 | 5.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM MES, pH 6.5, 1.6 M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
