4H0C
Crystal structure of phospholipase/Carboxylesterase from Dyadobacter fermentans DSM 18053
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.157, 40.251, 67.256 |
| Unit cell angles | 72.77, 78.37, 76.09 |
Refinement procedure
| Resolution | 63.600 - 1.620 |
| R-factor | 0.1391 |
| Rwork | 0.136 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.293 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.600 | 1.630 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.089 | 0.430 |
| Number of reflections | 47679 | |
| <I/σ(I)> | 22 | 2.15 |
| Completeness [%] | 96.8 | 94 |
| Redundancy | 96.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1 M tri sodium citrate, 20% PEG4000, 5% iso-propanol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
