4H02

Crystal structure of P. falciparum Lysyl-tRNA synthetase

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	141.334, 59.136, 297.222
Unit cell angles	90.00, 97.47, 90.00

Refinement procedure

Resolution	48.702 - 2.905
R-factor	0.2279
Rwork	0.226
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.278
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.960
High resolution limit [Å]	2.905	2.905
Rmerge	0.108	0.403
Number of reflections	103791
<I/σ(I)>	11.3
Completeness [%]	95.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M Bis-Tris (6.5 pH), 2%v/v Tascimate (6.0 pH) and 20%w/v PEG-3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K