4H00
The crystal structure of mon-Zn dihydropyrimidinase from Tetraodon nigroviridis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4r |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 160.998, 160.998, 94.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.17219 |
Rwork | 0.170 |
R-free | 0.20427 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.927 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | |
Number of reflections | 40386 | |
Completeness [%] | 99.2 | 99.6 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | lithium sulfate monohydrate, PEG 4000 (12%, w/v), tris-sodium citrate dihydrate (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | lithium sulfate monohydrate, PEG 4000 (12%, w/v), tris-sodium citrate dihydrate (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 291K |