4GZY
Crystal structures of bacterial RNA Polymerase paused elongation complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 207.210, 207.210, 203.223 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.770 - 3.505 |
| R-factor | 0.2661 |
| Rwork | 0.263 |
| R-free | 0.32170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.950 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 9.480 | 3.500 |
| Rmerge | 0.149 | 0.053 | |
| Number of reflections | 63554 | ||
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 100.0 | 100 | 99.9 |
| Redundancy | 9.9 | 13.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 3.5-6% PEG 8000, 0.1M MES pH 6.1-6.3, 0.3M LiCl, 10mM MgCl2, vapor diffusion, hanging drop, temperature 295K |
