4GZU
Crystal structure of the DH-PH-PH domain of FARP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.394, 85.248, 103.451 |
| Unit cell angles | 90.00, 118.76, 90.00 |
Refinement procedure
| Resolution | 45.343 - 3.200 |
| R-factor | 0.2346 |
| Rwork | 0.232 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 4GYV 1fhw and 2D9Y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.005 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
| Rmerge | 0.111 | 0.035 | 0.750 |
| Number of reflections | 23322 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 99.7 | 97.9 | 99.9 |
| Redundancy | 4.1 | 3.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MMT, 0.2 M LiSO4, 20% PEG3350, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
