4GZK
Structure and interactions of the RNA-dependent RNA polymerase from bacteriophage phi12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 134.805, 72.320, 96.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.850 - 1.690 |
| R-factor | 0.13139 |
| Rwork | 0.130 |
| R-free | 0.16718 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.533 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.700 |
| Rmerge | 0.069 | 0.411 |
| Number of reflections | 105656 | |
| <I/σ(I)> | 39.5 | 6.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.3 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 4 M Na formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
