4GZA

Complex of mouse Plexin A2 - Semaphorin 3A - Neuropilin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91730
Spacegroup name	C 1 2 1
Unit cell lengths	191.632, 293.604, 252.176
Unit cell angles	90.00, 106.38, 90.00

Refinement procedure

Resolution	125.506 - 7.000
R-factor	0.2847
Rwork	0.285
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3okt 1q47 4gz9
RMSD bond length	0.014
RMSD bond angle	1.202
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	126.000
High resolution limit [Å]	7.000
Number of reflections	20176
Completeness [%]	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	70mM HEPES, pH 7.5, 1.4% w/v PEG 400, 12% v/v glycerol, 1.4M ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 293K
2	VAPOR DIFFUSION, SITTING DROP	7.5	293	70mM HEPES, pH 7.5, 1.4% w/v PEG 400, 12% v/v glycerol, 1.4M ammonium sulphate, 1% v/v ethyl acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K
3	VAPOR DIFFUSION, SITTING DROP	7.5	293	70mM HEPES, pH 7.5, 1.4% w/v PEG 400, 12% v/v glycerol, 1.4M ammonium sulphate, 8% v/v acetonitrile , VAPOR DIFFUSION, SITTING DROP, temperature 293K