4GZ9

Mouse Neuropilin-1, extracellular domains 1-4 (a1a2b1b2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9173
Spacegroup name	P 3 2 1
Unit cell lengths	245.359, 245.359, 47.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	81.000 - 2.700
R-factor	0.20007
Rwork	0.199
R-free	0.22279
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qqm 2qqk
RMSD bond length	0.009
RMSD bond angle	1.582
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	81.000
High resolution limit [Å]	2.700
Number of reflections	45127
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES, pH 7.5, 1.5M lithium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 293K