4GZ6

Crystal structure of human O-GlcNAc Transferase with UDP-5SGlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 3 2 1
Unit cell lengths	272.338, 272.338, 143.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.412 - 2.980
R-factor	0.1983
Rwork	0.197
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.560
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.140
High resolution limit [Å]	2.980	2.980
Number of reflections	122037
Completeness [%]	98.3	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	1.9M Ammonium Sulfate, 0.1M Tris pH 8.5, 1% Xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 298K