4GYT
Crystal structure of lpg0076 protein from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.847, 85.872, 103.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.789 - 2.047 |
| R-factor | 0.1713 |
| Rwork | 0.169 |
| R-free | 0.21290 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.203 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: dev_1096)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.047 | 2.050 |
| Rmerge | 0.075 | 0.752 |
| Number of reflections | 28918 | |
| <I/σ(I)> | 27.4 | 3.5 |
| Completeness [%] | 96.7 | 97.3 |
| Redundancy | 8.8 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Bis-Tris, 0.2M ammonium acetate, 27% PEG3350, 5% Jeff M-600, 1/280 trypsin , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
