4GYO

Crystal Structure of Rap Protein Complexed with Competence and Sporulation Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	86.725, 86.725, 225.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.000 - 2.160
R-factor	0.1913
Rwork	0.190
R-free	0.22510
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.160	2.160
Number of reflections	47237
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	18 mM Tris (pH 8.0), 133 mM KCl, 4 mM DTT, 294 mM magnesium chloride, 2% benzamidine hydrochloride, 9% PEG 3000, 100 mM sodium cacodylate (pH 6.4), VAPOR DIFFUSION, HANGING DROP, temperature 293K