4GYN
The E142L mutant of the amidase from Geobacillus pallidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-18 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 130.789, 130.789, 130.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.390 - 1.900 |
| R-factor | 0.1958 |
| Rwork | 0.194 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2plq |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.008 |
| Data reduction software | d*TREK (9.9.9.5L) |
| Data scaling software | d*TREK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.390 | 53.390 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.205 | 0.122 | 0.516 |
| Total number of observations | 16491 | 14454 | |
| Number of reflections | 29066 | ||
| <I/σ(I)> | 5 | 9.9 | 2.3 |
| Completeness [%] | 94.6 | 95.5 | 93.7 |
| Redundancy | 5.66 | 5.11 | 5.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 1.2M sodium citrate, 0.4M sodium chloride, 0.1M sodium acetate, pH 5.6, vapor diffusion, hanging drop, temperature 294K |
