4GYM
Crystal structure of Glyoxalase/bleomycin resistance protein/dioxygenase from Conexibacter woesei DSM 14684
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.943, 58.943, 164.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.200 - 1.560 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.18690 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.362 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.115 | 0.939 |
| Number of reflections | 41562 | |
| <I/σ(I)> | 24.9 | 1.8 |
| Completeness [%] | 97.7 | 99.4 |
| Redundancy | 7.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M MgCl2, 0.1M This-Cl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
