4GYJ
Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-11 |
| Detector | RAYONIX MX-300 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.450, 73.390, 83.430 |
| Unit cell angles | 98.65, 110.14, 92.43 |
Refinement procedure
| Resolution | 38.610 - 1.650 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bmx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.610 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.059 | 0.622 |
| Number of reflections | 147692 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 95.8 | 94.3 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 296 | 22% PEG3350, pH 7.5 , VAPOR DIFFUSION, HANGING DROP, temperature 296K |
