4GYC

Structure of the SRII(D75N mutant)/HtrII Complex in I212121 space group ("U" shape)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-27
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.934
Spacegroup name	I 21 21 21
Unit cell lengths	49.465, 113.674, 126.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.229 - 2.050
R-factor	0.2037
Rwork	0.203
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2s
RMSD bond length	0.011
RMSD bond angle	1.130
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.229	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	22785
Completeness [%]	99.9	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.1	295.5	1M Na/KPi, 0.3M trehalose, pH 5.1, Lipidic cubic phase, temperature 295.5K
1	LIPIDIC CUBIC PHASE	5.1	295.5	1M Na/KPi, 0.3M trehalose, pH 5.1, Lipidic cubic phase, temperature 295.5K