4GY4
Role of the biradical intermediate observed during the turnover of SLAC: A two-domain laccase from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 178.440, 178.440, 177.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.800 - 2.670 |
| R-factor | 0.21515 |
| Rwork | 0.215 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.925 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.800 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Number of reflections | 82018 | |
| <I/σ(I)> | 5.7 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 35-45% polyethylene glycol-550-monomethyl ether, 0.15-0.5M NaCl, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
