4GXZ
Crystal structure of a periplasmic thioredoxin-like protein from Salmonella enterica serovar Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.107, 90.998, 83.309 |
| Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
| Resolution | 48.689 - 2.040 |
| R-factor | 0.2018 |
| Rwork | 0.199 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gyk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.003 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.065 | 0.479 |
| Number of reflections | 106150 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.45 | 7.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 298 | 18-27% (w/v) PEG 4000, 0.1M Tris buffer pH 8.2 , VAPOR DIFFUSION, temperature 298K |
