4GXV
Crystal structure of anti-influenza virus antibody 1F1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2009-07-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.789, 99.461, 175.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.096 - 1.449 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2FB4 AND 1RZF |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.449 | 1.449 |
| Number of reflections | 160778 | |
| <I/σ(I)> | 10.4 | 2.4 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 6.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG4000, 200 mM sodium phosphate dibasic, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
