4GXR

Structure of ATP bound RpMatB-BxBclM chimera B3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 61
Unit cell lengths	173.155, 173.155, 47.938
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.14982
Rwork	0.148
R-free	0.17787
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fut
RMSD bond length	0.005
RMSD bond angle	1.169
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.061	0.058
<I/σ(I)>		6.086
Completeness [%]	99.9	100
Redundancy		6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	triethanolamine (100 mM, pH 8.0), with magnesium sulfate (50 mM), monomethyl polyethylene glycol 5000 (21 %, w/v), and glycerol (4 %, w/v), VAPOR DIFFUSION, HANGING DROP, temperature 298K