4GXJ
R283K DNA polymerase beta ternary complex with a templating 8OG and incoming dCTP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-13 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.084, 77.432, 55.125 |
| Unit cell angles | 90.00, 114.45, 90.00 |
Refinement procedure
| Resolution | 21.060 - 2.200 |
| R-factor | 0.237 |
| Rwork | 0.231 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2FMS.PDB |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.153 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.196 | 2.200 |
| Rmerge | 0.097 | 0.579 |
| <I/σ(I)> | 14.7 | 2.95 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 291 | 50mM Imidazole, 350mM Sodium Acetate, 18% Peg3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |
