4GXE
T. vulcanus Phycocyanin crystallized in 4M Urea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 63 |
| Unit cell lengths | 153.750, 153.750, 39.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3018 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 18.710 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.107 |
| Number of reflections | 10963 |
| <I/σ(I)> | 7.2 |
| Completeness [%] | 98.9 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 0.8M ammonium sulfate, 20mM Tris pH 8 mixed 1:1 with 10mg/ml protein + 4M urea, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
