4GXD
Crystal structure of D48V mutant of human GLTP bound with 12:0 disulfatide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9754 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.253, 47.317, 63.887 |
| Unit cell angles | 90.00, 126.07, 90.00 |
Refinement procedure
| Resolution | 14.760 - 2.100 |
| R-factor | 0.18634 |
| Rwork | 0.183 |
| R-free | 0.24878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 12893 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15% PEG 3350, 0.1M MES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
