4GXB

Structure of the SNX17 atypical FERM domain bound to the NPxY motif of P-selectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-11
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95369
Spacegroup name	P 31 2 1
Unit cell lengths	93.754, 93.754, 91.308
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.597 - 1.800
R-factor	0.1785
Rwork	0.177
R-free	0.20380
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	0.947
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.900
High resolution limit [Å]	1.800
Rmerge	0.156
Number of reflections	43434
<I/σ(I)>	18.3
Completeness [%]	100.0
Redundancy	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M sodium acetate, 0.2M magnesium chloride, 15% PEG4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M sodium acetate, 0.2M magnesium chloride, 15% PEG4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K