4GX6
AMP Complexes of Porcine Liver Fructose-1,6-bisphosphatase with Mutation E192Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-11 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.923, 165.341, 79.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.560 - 2.500 |
| R-factor | 0.21405 |
| Rwork | 0.211 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyj |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.913 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK (9.7L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.220 | 45.220 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.143 | 0.075 | 0.331 |
| Number of reflections | 25867 | ||
| <I/σ(I)> | 5.8 | 10.7 | 3 |
| Completeness [%] | 92.3 | 88.5 | 94.1 |
| Redundancy | 3.52 | 3.78 | 3.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |
