4GX6

AMP Complexes of Porcine Liver Fructose-1,6-bisphosphatase with Mutation E192Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-11-11
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	59.923, 165.341, 79.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.560 - 2.500
R-factor	0.21405
Rwork	0.211
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eyj
RMSD bond length	0.022
RMSD bond angle	1.913
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK (9.7L)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.220	45.220	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.143	0.075	0.331
Number of reflections	25867
<I/σ(I)>	5.8	10.7	3
Completeness [%]	92.3	88.5	94.1
Redundancy	3.52	3.78	3.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	7.5	298	PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K