4GX4
AMP Complexes of Porcine Liver Fructose-1,6-bisphosphatase with mutation R22M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-07-20 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.776, 166.535, 79.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.240 - 2.500 |
R-factor | 0.21648 |
Rwork | 0.214 |
R-free | 0.27111 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eyj |
RMSD bond length | 0.021 |
RMSD bond angle | 1.903 |
Data reduction software | d*TREK (9.7L) |
Data scaling software | d*TREK (9.7L) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.240 | 32.240 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.139 | 0.070 | 0.427 |
Number of reflections | 27370 | ||
<I/σ(I)> | 5.9 | 10.8 | 2.4 |
Completeness [%] | 95.6 | 89 | 96.3 |
Redundancy | 3.67 | 3.79 | 3.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |