4GX3
Product Complexes of Porcine Liver Fructose-1,6-bisphosphatase with mutation R22M Reveal a T-state Conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-21 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.249, 165.012, 79.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.920 - 2.250 |
| R-factor | 0.21453 |
| Rwork | 0.212 |
| R-free | 0.26953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyj |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.923 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK (9.7L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.920 | 35.920 | 2.330 |
| High resolution limit [Å] | 2.250 | 4.840 | 2.250 |
| Rmerge | 0.073 | 0.040 | 0.299 |
| Number of reflections | 32141 | ||
| <I/σ(I)> | 12.2 | 24.5 | 2.6 |
| Completeness [%] | 85.2 | 81.2 | 69.2 |
| Redundancy | 3.45 | 4.2 | 1.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |
