4GWZ

Crystal Structure of AMP Complexes of Porcine Liver Fructose-1,6-bisphosphatase with Restrained Subunit Pair Rotation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-10-23
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	59.776, 166.064, 79.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.840 - 2.600
R-factor	0.21444
Rwork	0.211
R-free	0.27196
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.897
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK (9.7L)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.460	41.460	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.086	0.042	0.338
Number of reflections	24685
<I/σ(I)>	8	16.3	2.6
Completeness [%]	97.8	92.5	99.6
Redundancy	3.63	3.61	3.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	7.5	298	PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K