4GWZ
Crystal Structure of AMP Complexes of Porcine Liver Fructose-1,6-bisphosphatase with Restrained Subunit Pair Rotation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-10-23 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.776, 166.064, 79.871 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.840 - 2.600 |
R-factor | 0.21444 |
Rwork | 0.211 |
R-free | 0.27196 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.897 |
Data reduction software | d*TREK (9.7L) |
Data scaling software | d*TREK (9.7L) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.460 | 41.460 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.086 | 0.042 | 0.338 |
Number of reflections | 24685 | ||
<I/σ(I)> | 8 | 16.3 | 2.6 |
Completeness [%] | 97.8 | 92.5 | 99.6 |
Redundancy | 3.63 | 3.61 | 3.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |