4GWY
Crystal Structure of AMP Complexes of Porcine Liver Fructose-1,6-bisphosphatase with Blocked Subunit Pair Rotation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-08 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.261, 82.571, 168.885 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.090 - 3.000 |
| R-factor | 0.21646 |
| Rwork | 0.214 |
| R-free | 0.26558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yyz |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.744 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK (9.7L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.550 | 39.550 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.137 | 0.056 | 0.401 |
| Number of reflections | 7139 | ||
| <I/σ(I)> | 7 | 14.1 | 2.5 |
| Completeness [%] | 91.5 | 86.7 | 94.3 |
| Redundancy | 3.7 | 3.66 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |
