4GWW
Crystal Structure of product complexes of Porcine Liver Fructose-1,6-bisphosphatase with blocked subunit pair rotation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-10-18 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.444, 82.629, 165.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.855 - 3.200 |
| R-factor | 0.24482 |
| Rwork | 0.242 |
| R-free | 0.30014 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cnq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.738 |
| Data reduction software | d*TREK (9.7L) |
| Data scaling software | d*TREK (9.7L) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.855 | 38.370 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.450 | 3.000 |
| Rmerge | 0.169 | 0.073 | 0.488 |
| Number of reflections | 7145 | ||
| <I/σ(I)> | 4.5 | 11.2 | 1.6 |
| Completeness [%] | 93.1 | 88.1 | 96.6 |
| Redundancy | 3.02 | 3.32 | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.5 | 298 | PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K |
